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A transcriptional activator is a protein (transcription factor) that increases gene transcription of a gene or set of genes. Most activators are DNA-binding proteins that bind to enhancers or promoter-proximal elements.
Most activators function by binding sequence-specifically to a DNA site located in or near a promoter and making protein–protein interactions with the general transcription machinery (RNA polymerase and general transcription factors), thereby facilitating the binding of the general transcription machinery to the promoter. The DNA site bound by the activator is referred to as an 'activator site'. The part of the activator that makes protein–protein interactions with the general transcription machinery is referred to as an 'activating region'. The part of the general transcription machinery that makes protein–protein interactions with the activator is referred to as an 'activation target'.
Example[edit]
The catabolite activator protein (CAP; also known as cAMP receptor protein, CRP) activates transcription at the lac operon of the bacterium Escherichia coli.[1]Cyclic adenosine monophosphate (cAMP) is produced during glucose starvation, binds to CAP, causes a conformational change that allows CAP to bind to a DNA site located adjacent to the lac promoter. CAP then makes a direct protein–protein interaction with RNA polymerase that recruits RNA polymerase to the lac promoter.
lac operon in detail
See also[edit]
References[edit]
^Busby S., Ebright RH. (2001). 'Transcription activation by catabolite activator protein (CAP)'. J. Mol. Biol. 293 (2): 199–213. doi:10.1006/jmbi.1999.3161. PMID10550204.
Retrieved from 'https://en.wikipedia.org/w/index.php?title=Activator_(genetics)&oldid=931108907'
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